Hotspot

petA-psbJ

LSC · 60891-61570 · 680 bp

Back to Erythrina

Pi 0.0098

MarkerSeek score 67.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 10
pi: 0.0098
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCAGGATTGGGCTCATCC	50.0	50.0	57.8	57.3	812-838 bp	1.0	74.9
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCAGGATTGGGCTCATCC	55.0	50.0	58.3	57.3	817-843 bp	1.0	74.4
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCAGGATTGGGCTCATCC	55.0	50.0	58.4	57.3	821-847 bp	1.0	73.9
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	ATTCAGGATTGGGCTCATCC	50.0	50.0	58.9	57.3	813-839 bp	1.0	69.8
petA-psbJ_p5	ATCCGTTACGTGTCCAAGGT	ATTCAGGATTGGGCTCATCC	50.0	50.0	59.0	57.3	820-846 bp	1.0	68.6