Hotspot

trnR-UCU-trnG-UCC

LSC · 51183-51456 · 274 bp

Back to Erythrina

Pi 0.0243

MarkerSeek score 65.60

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 10
pi: 0.0243
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	TGCGCAAACATATGCAAACA	AGCCTTCCAAGCTAACGATG	40.0	50.0	57.6	58.0	413-447 bp	1.0	78.9
trnR-UCU-trnG-UCC_p2	TGCGCAAACATATGCAAACA	CCTAGCCTTCCAAGCTAACG	40.0	55.0	57.6	58.1	416-450 bp	1.0	78.7
trnR-UCU-trnG-UCC_p3	TGCGCAAACATATGCAAACA	CCCTAGCCTTCCAAGCTAAC	40.0	55.0	57.6	57.7	417-451 bp	1.0	76.5
trnR-UCU-trnG-UCC_p4	TGCGCAAACATATGCAAACA	CTAACGATGCGGGTTCGATT	40.0	50.0	57.6	58.4	402-436 bp	1.0	75.8
trnR-UCU-trnG-UCC_p5	TGCGCAAACATATGCAAACA	TAACGATGCGGGTTCGATTC	40.0	50.0	57.6	58.4	401-435 bp	1.0	75.7