Hotspot

atpA-trnR-UCU

LSC · 50903-51110 · 208 bp

Back to Erythrina

Pi 0.0353

MarkerSeek score 72.50

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 11
pi: 0.0353
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	315-343 bp	1.0	69.2
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	319-347 bp	1.0	69.2
atpA-trnR-UCU_p3	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	305-333 bp	1.0	68.0
atpA-trnR-UCU_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	314-342 bp	1.0	66.8
atpA-trnR-UCU_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	317-345 bp	1.0	66.5