Hotspot

atpI-atpH

LSC · 46496-47372 · 877 bp

Back to Erythrina

Pi 0.0125

MarkerSeek score 66.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 16
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.3	58.1	957-981 bp	1.0	74.6
atpI-atpH_p2	GCTGCGGCTTATATAGGTGAA	GCAGCAATAACAGAAGCAGC	47.6	50.0	58.0	58.1	957-981 bp	1.0	72.2
atpI-atpH_p3	GCTGCGGCTTATATAGGTGA	AATCCCTTCTACAGCTTGCC	50.0	50.0	57.3	57.6	1036-1060 bp	1.0	71.9
atpI-atpH_p4	GCTGCGGCTTATATAGGTGA	AGGTCCAATAGAAGCAAGCC	50.0	50.0	57.3	57.6	991-1015 bp	1.0	71.9
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCT	50.0	50.0	57.3	57.6	989-1013 bp	1.0	71.9