Hotspot

trnC-GCA-rpoB

LSC · 32984-34100 · 1117 bp

Back to Erythrina

Pi 0.0167

MarkerSeek score 60.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 26
pi: 0.0167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	ATTCCTTCATTTCCACCCCC	50.0	50.0	58.1	57.4	1185-1213 bp	1.0	79.3
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	CCTTCATTTCCACCCCCAAC	50.0	55.0	58.1	58.7	1182-1210 bp	1.0	77.9
trnC-GCA-rpoB_p3	CCGGATTTGAACTGGGGAAA	CTTCATTTCCACCCCCAACC	50.0	55.0	58.1	58.7	1181-1209 bp	1.0	77.9
trnC-GCA-rpoB_p4	TTATTGATCAGGCGACACCC	ATTCCTTCATTTCCACCCCC	50.0	50.0	57.7	57.4	1203-1231 bp	1.0	77.3
trnC-GCA-rpoB_p5	AAAAGGATTTGCAGTCCCCC	ATTCCTTCATTTCCACCCCC	50.0	50.0	58.4	57.4	1167-1195 bp	1.0	77.2