Hotspot

psbM-petN

LSC · 31188-31908 · 721 bp

Back to Erythrina

Pi 0.0134

MarkerSeek score 66.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 14
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	38.1	45.0	55.2	58.0	873-876 bp	1.0	52.1
psbM-petN_p2	TGCTACTGCACTGTTTATTCT	AGTAAGTCTTGCTTGGGCTG	38.1	50.0	55.2	57.8	879-882 bp	1.0	50.6
psbM-petN_p3	TGCTACTGCACTGTTTATTCTAGT	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	58.0	58.0	873-876 bp	1.0	49.8
psbM-petN_p4	ACTGCACTGTTTATTCTAGTTCCT	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	57.9	58.0	869-872 bp	1.0	49.2
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	CCCTCGTAGTATGGGGAAGA	38.1	55.0	55.2	57.6	829-832 bp	1.0	49.2