Hotspot

trnG-GCC-psbZ

LSC · 24190-24692 · 503 bp

Back to Erythrina

Pi 0.0497

MarkerSeek score 84.70

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 36
pi: 0.0497
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTCTTGAAGGTTGGTCGAGT	50.0	50.0	57.8	57.2	654-939 bp	1.0	79.5
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TCTCTTGAAGGTTGGTCGAG	50.0	50.0	57.8	56.9	655-940 bp	1.0	77.2
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	CTTCTCTTGAAGGTTGGTCGA	50.0	47.6	57.8	57.6	657-942 bp	1.0	75.0
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	TTCTCTTGAAGGTTGGTCGAG	50.0	47.6	57.8	57.6	656-941 bp	1.0	75.0
trnG-GCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	TGAAGGTTGGTCGAGTAACA	50.0	45.0	57.8	56.4	650-935 bp	1.0	73.5