Hotspot

trnK-UUU-rbcL

LSC · 4297-5230 · 934 bp

Back to Erythrina

Pi 0.0222

MarkerSeek score 59.80

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 31
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGAGTCGCTCTTAAAAGCCG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1056-1114 bp	1.0	82.7
trnK-UUU-rbcL_p2	AGTCGCTCTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1054-1112 bp	1.0	82.7
trnK-UUU-rbcL_p3	AGAGTCGCTCTTAAAAGCCG	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.0	58.0	1071-1129 bp	1.0	82.6
trnK-UUU-rbcL_p4	AGTCGCTCTTAAAAGCCGAG	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.0	58.0	1069-1127 bp	1.0	82.6
trnK-UUU-rbcL_p5	GAGTCGCTCTTAAAAGCCGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1055-1113 bp	1.0	82.6