Hotspot

rpl32-trnL-UAG

SSC · 112765-113214 · 450 bp

Back to Erythranthe

Pi 0.0383
MarkerSeek score 68.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
450
variable_sites
63
pi
0.0383
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAGGAAGGGAGATTGGGC TTTAGGAGATGACATGCCGC 50.0 50.0 57.8 58.0 655-987 bp 1.0 88.2
rpl32-trnL-UAG_p2 AAAAGGAAGGGAGATTGGGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 600-932 bp 1.0 88.0
rpl32-trnL-UAG_p3 CAGCGTTAAAGGCGTTTTCC TTTAGGAGATGACATGCCGC 50.0 50.0 58.3 58.0 636-968 bp 1.0 87.6
rpl32-trnL-UAG_p4 AAAAGGAAGGGAGATTGGGC AGCAAAGCCCTGGGTAAAAA 50.0 45.0 57.8 57.9 1088-1412 bp 1.0 86.9
rpl32-trnL-UAG_p5 AAAAGGAAGGGAGATTGGGC TGATCCACATAAAAGGGCCG 50.0 50.0 57.8 57.9 1211-1535 bp 1.0 86.7