Hotspot

ndhF-rpl32

SSC · 112057-112587 · 531 bp

Back to Erythranthe

Pi 0.0292

MarkerSeek score 61.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 531
variable_sites: 56
pi: 0.0292
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGAAGGAAGGGTA	TGATGCAGAAGTACGTTTTT	50.0	35.0	57.6	53.5	615-636 bp	1.0	48.4
ndhF-rpl32_p2	TGGAAGTGGAAGGAAGGGTA	TTGATGCAGAAGTACGTTTT	50.0	35.0	57.6	53.5	616-637 bp	1.0	48.4
ndhF-rpl32_p3	TGGAAGTGGAAGGAAGGGTA	TTTGATGCAGAAGTACGTTT	50.0	35.0	57.6	53.5	617-638 bp	1.0	48.4
ndhF-rpl32_p4	TGGAAGTGGAAGGAAGGGTA	TTTTGATGCAGAAGTACGTT	50.0	35.0	57.6	53.5	618-639 bp	1.0	48.4
ndhF-rpl32_p5	TGGAAGTGGAAGGAAGGGTA	TTTTTGATGCAGAAGTACGT	50.0	35.0	57.6	53.5	619-640 bp	1.0	48.4