Hotspot

ndhC-trnV-UAC

LSC · 50970-52158 · 1189 bp

Back to Erythranthe

Pi 0.0234
MarkerSeek score 63.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1189
variable_sites
110
pi
0.0234
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCAGAAATG AGAAGGTCTACGGTTCGAGT 47.6 50.0 56.9 57.8 1267-1324 bp 1.0 72.3
ndhC-trnV-UAC_p2 GGGGCTAAAATCCCAGAAATGA AGAAGGTCTACGGTTCGAGT 45.5 50.0 58.4 57.8 1267-1324 bp 1.0 70.3
ndhC-trnV-UAC_p3 GGGGCTAAAATCCCAGAAATG GAAGGTCTACGGTTCGAGTC 47.6 55.0 56.9 57.5 1266-1323 bp 1.0 69.5
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCAGAAATG GTTCGAGTCCGTATAGCCCT 47.6 55.0 56.9 58.7 1255-1312 bp 1.0 68.5
ndhC-trnV-UAC_p5 GGGGCTAAAATCCCAGAAAT AGAAGGTCTACGGTTCGAGT 45.0 50.0 55.3 57.8 1267-1324 bp 1.0 67.8