Hotspot

atpH-atpI

LSC · 14199-15222 · 1024 bp

Back to Erythranthe

Pi 0.0206

MarkerSeek score 59.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 84
pi: 0.0206
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAACTCCAGGCCCAATAGAC	GGCAAATAGGGGGTTTCCAA	55.0	50.0	58.0	58.1	1716-1774 bp	1.0	88.0
atpH-atpI_p2	CAACTCCAGGCCCAATAGAC	TTGGCAAATAGGGGGTTTCC	55.0	50.0	58.0	58.1	1718-1776 bp	1.0	88.0
atpH-atpI_p3	CAAATCACCCCTTCCCGTAG	GGCAAATAGGGGGTTTCCAA	55.0	50.0	58.0	58.1	2029-2079 bp	0.941176	85.4
atpH-atpI_p4	CAAATCACCCCTTCCCGTAG	TTGGCAAATAGGGGGTTTCC	55.0	50.0	58.0	58.1	2031-2081 bp	0.941176	85.4
atpH-atpI_p5	CAAATCACCCCTTCCCGTAG	TTCGGAACCCACAAACCATT	55.0	45.0	58.0	57.9	1939-1989 bp	0.941176	85.3