Hotspot

trnS-GCU-trnG-UCC

LSC · 8915-9598 · 684 bp

Back to Erythranthe

Pi 0.0271

MarkerSeek score 62.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 66
pi: 0.0271
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	736-764 bp	1.0	58.2
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	731-759 bp	1.0	57.4
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	AACGAATCACACTTTTACCACT	50.0	36.4	57.6	56.2	737-765 bp	1.0	56.2
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	AACGAATCACACTTTTACCACT	55.0	36.4	58.5	56.2	732-760 bp	1.0	55.5
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.2	55.5	732-760 bp	1.0	54.8