Hotspot

trnK-UUU-rps16

LSC · 4411-5316 · 906 bp

Back to Erythranthe

Pi 0.0287
MarkerSeek score 69.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
906
variable_sites
93
pi
0.0287
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAGCGGAAGTTTTTAAGGAACT 50.0 34.8 58.2 57.2 956-1008 bp 1.0 59.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAGCGGAAGTTTTTAAGGAACT 50.0 34.8 58.2 57.2 955-1007 bp 1.0 59.4
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AGCGGAAGTTTTTAAGGAAC 50.0 40.0 58.2 54.1 954-1006 bp 1.0 59.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGCGGAAGTTTTTAAGGAAC 50.0 40.0 58.2 54.1 953-1005 bp 1.0 59.2
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC AAAGCGGAAGTTTTTAAGGAACT 50.0 34.8 58.3 57.2 970-1022 bp 1.0 58.5