Hotspot

ndhF

SSC · 109996-112236 · 2241 bp

Back to Erysimum

Pi 0.00308

MarkerSeek score 42.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 22
pi: 0.00308
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATGATGAATTCCACGTTCG	ACAAACCAGTGAATAAGAAATAA	47.6	26.1	57.4	52.3	2383 bp	1.0	40.7
ndhF_p2	TCCGAGTGAATGGAAAAGACA	ACAAACCAGTGAATAAGAAATAA	42.9	26.1	57.2	52.3	2414 bp	1.0	40.7
ndhF_p3	GGATGATGAATTCCACGTTCG	ATACAAACCAGTGAATAAGAAAT	47.6	26.1	57.4	52.1	2385 bp	1.0	40.7
ndhF_p4	GGATGATGAATTCCACGTTCG	ACAAACCAGTGAATAAGAAATAAT	47.6	25.0	57.4	52.9	2383 bp	1.0	40.7
ndhF_p5	TCCGAGTGAATGGAAAAGACA	ATACAAACCAGTGAATAAGAAAT	42.9	26.1	57.2	52.1	2416 bp	1.0	40.7