Hotspot

psaJ-rpl33

LSC · 66310-66759 · 450 bp

Back to Erysimum

Pi 0.00307

MarkerSeek score 51.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 6
pi: 0.00307
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGGTTCGTTAGCGGGTTTAT	TCATTTCGAGCACAACTGGT	50.0	45.0	58.4	57.7	574-588 bp	1.0	78.7
psaJ-rpl33_p2	CGGTTCGTTAGCGGGTTTAT	TTTCGAGCACAACTGGTACA	50.0	45.0	58.4	57.4	571-585 bp	1.0	75.9
psaJ-rpl33_p3	CCGTAGCACCGGTACTAAGT	TCATTTCGAGCACAACTGGT	55.0	45.0	58.9	57.7	605-619 bp	1.0	74.1
psaJ-rpl33_p4	CGGTTCGTTAGCGGGTTTAT	TCGAGCACAACTGGTACATT	50.0	45.0	58.4	57.2	569-583 bp	1.0	74.1
psaJ-rpl33_p5	CGGTTCGTTAGCGGGTTTAT	TTCGAGCACAACTGGTACAT	50.0	45.0	58.4	57.2	570-584 bp	1.0	74.1