Hotspot

petA-psbJ

LSC · 61945-62926 · 982 bp

Back to Erysimum

Pi 0.00339

MarkerSeek score 55.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 11
pi: 0.00339
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.0	58.1	1176-1193 bp	1.0	80.0
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.2	58.1	1173-1190 bp	1.0	78.8
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1171-1188 bp	1.0	77.4
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.4	58.1	1170-1187 bp	1.0	77.1
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.5	58.1	1166-1183 bp	1.0	76.6