Hotspot

ycf4-cemA

LSC · 59646-60066 · 421 bp

Back to Erysimum

Pi 0.00591

MarkerSeek score 46.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 6
pi: 0.00591
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AGGTCAAGGGGCTATTCCTT	AGAGAGATCAACCAGGGCAA	50.0	50.0	58.0	58.3	609-610 bp	1.0	78.4
ycf4-cemA_p2	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.0	58.3	533-534 bp	1.0	78.3
ycf4-cemA_p3	AGGTCAAGGGGCTATTCCTT	TCAACCAGGGCAAAAAGACT	50.0	45.0	58.0	57.5	602-603 bp	1.0	77.5
ycf4-cemA_p4	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	45.0	45.0	58.0	57.5	526-527 bp	1.0	77.4
ycf4-cemA_p5	AGGTCAAGGGGCTATTCCTT	GAGAGATCAACCAGGGCAAA	50.0	50.0	58.0	57.5	608-609 bp	1.0	77.1