Hotspot

rbcL-accD

LSC · 55766-56453 · 688 bp

Back to Erysimum

Pi 0.00773

MarkerSeek score 59.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 16
pi: 0.00773
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	847-884 bp	1.0	81.8
rbcL-accD_p2	CGTGAAGCTTGCAAATGGAG	GCTCGCCTCTGTATTCCAAT	50.0	50.0	58.0	57.8	845-882 bp	1.0	80.3
rbcL-accD_p3	CGTGAAGCTTGCAAATGGAG	TGCTTTACTTAGCTCGCCTC	50.0	50.0	58.0	57.7	856-893 bp	1.0	79.9
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TAGCTCGCCTCTGTATTCCA	55.0	50.0	57.7	57.9	830-867 bp	1.0	79.7
rbcL-accD_p5	AAGCTTGCAAATGGAGTCCT	TAGCTCGCCTCTGTATTCCA	45.0	50.0	57.7	57.9	843-880 bp	1.0	79.6