Hotspot

trnD-GAC-trnY-UAC

LSC · 29836-30276 · 441 bp

Back to Erysimum

Pi 0.00348

MarkerSeek score 51.50

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 6
pi: 0.00348
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	TGATGTATTGAATCCGCCGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.1	57.8	571-572 bp	1.0	78.1
trnD-GAC-trnY-UAC_p2	TGATGTATTGAATCCGCCGG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.1	57.8	570-571 bp	1.0	78.1
trnD-GAC-trnY-UAC_p3	TGATGTATTGAATCCGCCGG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.1	58.4	592-593 bp	1.0	77.2
trnD-GAC-trnY-UAC_p4	TGATGTATTGAATCCGCCGG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.1	57.6	591-592 bp	1.0	77.0
trnD-GAC-trnY-UAC_p5	TGATGTATTGAATCCGCCGG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.1	58.4	625-626 bp	1.0	76.7