Hotspot

psbM-trnD-GAC

LSC · 28761-29761 · 1001 bp

Back to Erysimum

Pi 0.0038
MarkerSeek score 57.40
Primer available yes
Label psbM-trnD-GAC

Feature Metadata

label_name
psbM-trnD-GAC
feature_type
igs
region
LSC
length_bp
1001
variable_sites
15
pi
0.0038
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GAC_p1 AGGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 1111-1122 bp 1.0 70.5
psbM-trnD-GAC_p2 ACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 45.0 50.0 56.9 58.5 1107-1118 bp 1.0 69.8
psbM-trnD-GAC_p3 GGAACGAGAATGAAGAGTGCA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.3 58.5 1110-1121 bp 1.0 68.3
psbM-trnD-GAC_p4 AACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 42.9 50.0 57.6 58.5 1108-1119 bp 1.0 67.6
psbM-trnD-GAC_p5 GGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.5 58.5 1110-1121 bp 1.0 67.1