Hotspot

rpoB-trnC-UGC

LSC · 26220-27296 · 1077 bp

Back to Erysimum

Pi 0.00249

MarkerSeek score 52.50

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1077
variable_sites: 12
pi: 0.00249
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1163-1166 bp	1.0	79.5
rpoB-trnC-UGC_p2	CCCTCTTTTCCATCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1160-1163 bp	1.0	79.5
rpoB-trnC-UGC_p3	CTCTTTTCCATCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1158-1161 bp	1.0	78.9
rpoB-trnC-UGC_p4	TCCCTCTTTTCCATCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1161-1164 bp	1.0	78.8
rpoB-trnC-UGC_p5	TTCCCTCTTTTCCATCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1162-1165 bp	1.0	78.8