Hotspot

ycf1

SSC · 126478-131847 · 5370 bp

Back to Eryngium

Pi 0.024

MarkerSeek score 53.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5370
variable_sites: 191
pi: 0.024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCGTGGATCTCTCAGTCC	TCCCAGAATTTGTGCCACTT	55.0	45.0	58.0	57.6	409 bp	0.666667	63.7
ycf1_p2	TCTCGTGGATCTCTCAGTCC	TTCCCAGAATTTGTGCCACT	55.0	45.0	58.0	57.6	410 bp	0.666667	63.7
ycf1_p3	TGGATCTCTCAGTCCAAGCA	TCCCAGAATTTGTGCCACTT	50.0	45.0	58.1	57.6	404 bp	0.666667	63.6
ycf1_p4	TGGATCTCTCAGTCCAAGCA	TTCCCAGAATTTGTGCCACT	50.0	45.0	58.1	57.6	405 bp	0.666667	63.6
ycf1_p5	AGCAAATAGTGTTCCCGCAA	TCCCAGAATTTGTGCCACTT	45.0	45.0	58.1	57.6	304 bp	0.666667	63.4