Hotspot

ndhC-trnV-UAC

LSC · 52340-53571 · 1232 bp

Back to Eryngium

Pi 0.0423

MarkerSeek score 80.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 75
pi: 0.0423
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	1136-1291 bp	1.0	48.9
ndhC-trnV-UAC_p2	GCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	34.8	55.0	54.7	58.1	1184-1339 bp	1.0	48.7
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACA	AACGCATGTTGGGTCTTTGA	31.8	45.0	53.9	58.3	1200-1355 bp	1.0	48.6
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	ACGCATGTTGGGTCTTTGAA	31.8	45.0	53.9	58.3	1199-1354 bp	1.0	48.6
ndhC-trnV-UAC_p5	GCCAAAATAGGAATAAGACTTGA	ACGCATGTTGGGTCTTTGAA	34.8	45.0	54.7	58.3	1247-1402 bp	1.0	48.5