Hotspot

trnE-UUC-trnT-GGU

LSC · 32570-33242 · 673 bp

Back to Eryngium

Pi 0.0387

MarkerSeek score 76.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 38
pi: 0.0387
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	777-788 bp	1.0	84.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GGCGTTACTCTACCGTTGAG	50.0	55.0	57.8	58.1	752-763 bp	1.0	83.9
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	774-785 bp	1.0	83.3
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	ATGGCGTTACTCTACCGTTG	50.0	50.0	57.8	57.7	754-765 bp	1.0	82.3
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	779-790 bp	1.0	82.2