Hotspot

psbM-trnD-GUC

LSC · 31469-32170 · 702 bp

Back to Eryngium

Pi 0.0328

MarkerSeek score 75.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 34
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	798-812 bp	1.0	79.9
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	797-811 bp	1.0	78.9
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	799-813 bp	1.0	78.1
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	801-815 bp	1.0	76.8
psbM-trnD-GUC_p5	ACTAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	804-818 bp	1.0	75.9