Hotspot

rpoC1-rpoB

LSC · 24798-24823 · 26 bp

Back to Eryngium

Pi 0.0513

MarkerSeek score 73.90

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 2
pi: 0.0513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	AGGCAGGATTTTATTGGCCC	TCCAGATTAATAGGAAGGAAGCT	50.0	39.1	58.2	56.5	139 bp	1.0	43.5
rpoC1-rpoB_p2	GGCAGGATTTTATTGGCCCA	TCCAGATTAATAGGAAGGAAGCT	50.0	39.1	58.5	56.5	138 bp	1.0	41.2
rpoC1-rpoB_p3	AGGCAGGATTTTATTGGCCC	TCCAGATTAATAGGAAGGAAGCTT	50.0	37.5	58.2	57.2	139 bp	1.0	41.2
rpoC1-rpoB_p4	AGGCAGGATTTTATTGGCCC	TCCAGATTAATAGGAAGGAAGC	50.0	40.9	58.2	55.0	139 bp	1.0	41.2
rpoC1-rpoB_p5	AGGCAGGATTTTATTGGCCC	TTCCAGATTAATAGGAAGGAAGCT	50.0	37.5	58.2	57.2	140 bp	1.0	41.1