Hotspot

atpF-atpH

LSC · 13882-14269 · 388 bp

Back to Eryngium

Pi 0.0438

MarkerSeek score 71.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 25
pi: 0.0438
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCCAAAGAAACGAAAGAATC	GCCTGGTTGTAGCATTAGCA	40.0	50.0	52.8	58.2	470-481 bp	1.0	45.2
atpF-atpH_p2	CCCAAAGAAACGAAAGAATC	TGGCCTGGTTGTAGCATTAG	40.0	50.0	52.8	57.6	472-483 bp	1.0	45.2
atpF-atpH_p3	CCCAAAGAAACGAAAGAATC	CGGAGGGAAAAATACGAGGT	40.0	50.0	52.8	57.3	536-547 bp	1.0	44.6
atpF-atpH_p4	CCCAAAGAAACGAAAGAATC	GCGGAGGGAAAAATACGAGG	40.0	55.0	52.8	58.7	537-548 bp	1.0	44.6
atpF-atpH_p5	CCCAAAGAAACGAAAGAATC	GGCGGAGGGAAAAATACGAG	40.0	55.0	52.8	58.7	538-549 bp	1.0	44.6