Hotspot

psbI-trnS-GCU

LSC · 8910-9042 · 133 bp

Back to Eryngium

Pi 0.0451

MarkerSeek score 73.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 9
pi: 0.0451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTCGTAATCCTGGACGTGAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.4	58.0	227-228 bp	1.0	78.1
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	225-226 bp	1.0	75.8
psbI-trnS-GCU_p3	TCGTAATCCTGGACGTGAAG	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	226-227 bp	1.0	75.8
psbI-trnS-GCU_p4	GTCGTAATCCTGGACGTGAA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.4	57.6	199-200 bp	1.0	75.7
psbI-trnS-GCU_p5	GTCGTAATCCTGGACGTGAA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.4	57.6	241-242 bp	1.0	74.9