Hotspot

rps16-trnQ-UUG

LSC · 6375-7742 · 1368 bp

Back to Eryngium

Pi 0.0364

MarkerSeek score 77.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1368
variable_sites: 74
pi: 0.0364
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1425-1469 bp	1.0	82.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.1	57.3	1407-1451 bp	1.0	81.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1434-1478 bp	1.0	81.5
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	1440-1484 bp	1.0	80.8
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GGGTTTTGGTCCCGGTATTC	55.0	55.0	58.4	58.5	1439-1483 bp	1.0	80.8