Hotspot

trnK-UUU-rps16

LSC · 4433-5299 · 867 bp

Back to Eryngium

Pi 0.0493
MarkerSeek score 83.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
867
variable_sites
62
pi
0.0493
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGAAAGGCGGAGGTTTTTAAGT 50.0 40.9 58.2 58.2 915-942 bp 1.0 70.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGAAAGGCGGAGGTTTTTAAGT 50.0 40.9 58.2 58.2 914-941 bp 1.0 70.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GAAAGGCGGAGGTTTTTAAGT 50.0 42.9 58.2 56.7 914-941 bp 1.0 69.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GAAAGGCGGAGGTTTTTAAGT 50.0 42.9 58.2 56.7 913-940 bp 1.0 69.2
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AGAAAGGCGGAGGTTTTTAAGT 50.0 40.9 58.3 58.2 924-951 bp 1.0 69.1