Hotspot

psaC

Genome · 126826-127071 · 246 bp

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Pi 0.519

MarkerSeek score 77.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: Genome
length_bp: 246
variable_sites: 120
pi: 0.519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AGGGCCTACAGAAAGTCCAT	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.0	58.0	971 bp	0.5	59.7
psaC_p2	GGTCCCGAGAAGCAAATGAT	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.0	58.0	1104 bp	0.5	59.5
psaC_p3	AGACTTGGACCACAAAACCC	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.9	58.0	690 bp	0.5	59.4
psaC_p4	AGGGCCTACAGAAAGTCCAT	GGCTGGCTATTGTTTCCTCA	50.0	50.0	58.0	57.9	964 bp	0.5	58.8
psaC_p5	GGTCCCGAGAAGCAAATGAT	GGCTGGCTATTGTTTCCTCA	50.0	50.0	58.0	57.9	1097 bp	0.5	58.6