Hotspot

ndhH

Genome · 131784-132965 · 1182 bp

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Pi 0.447

MarkerSeek score 73.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 312
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TATTCGGGGCCAATACGTTG	GTCAACGGCTGCTAGCTTAT	50.0	50.0	58.0	58.1	1597 bp	0.5	59.2
ndhH_p2	TATTCGGGGCCAATACGTTG	ATCCATATCAGGGAAGGGGG	50.0	55.0	58.0	57.9	1807 bp	0.5	59.1
ndhH_p3	TATTCGGGGCCAATACGTTG	CCATATCAGGGAAGGGGGAT	50.0	55.0	58.0	57.9	1805 bp	0.5	59.1
ndhH_p4	CCAACGGGAGCAATACTTGA	GTCAACGGCTGCTAGCTTAT	50.0	50.0	57.9	58.1	1831 bp	0.5	58.7
ndhH_p5	TCCAACGGGAGCAATACTTG	GTCAACGGCTGCTAGCTTAT	50.0	50.0	57.9	58.1	1832 bp	0.5	58.7