Hotspot

ndhG

Genome · 127858-128388 · 531 bp

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Pi 0.463

MarkerSeek score 70.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 531
variable_sites: 99
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACAATAGCCAGCCCAATAGC	TACCCGTTGTTGATTGGCAA	50.0	45.0	58.0	58.0	1599 bp	0.5	59.8
ndhG_p2	ACAATAGCCAGCCCAATAGC	TTACCCGTTGTTGATTGGCA	50.0	45.0	58.0	58.0	1600 bp	0.5	59.8
ndhG_p3	TGAGGAAACAATAGCCAGCC	TACCCGTTGTTGATTGGCAA	50.0	45.0	57.9	58.0	1606 bp	0.5	58.8
ndhG_p4	TGAGGAAACAATAGCCAGCC	TTACCCGTTGTTGATTGGCA	50.0	45.0	57.9	58.0	1607 bp	0.5	58.8
ndhG_p5	CAATAGCCAGCCCAATAGCT	TACCCGTTGTTGATTGGCAA	50.0	45.0	57.7	58.0	1598 bp	0.5	57.6