Hotspot

ndhD

Genome · 125199-126737 · 1539 bp

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Pi 0.485

MarkerSeek score 77.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1539
variable_sites: 559
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAATCCAAGAGGTTGCAGCA	GCTATTGGGCTGGCTATTGT	45.0	50.0	58.0	58.0	2442 bp	0.5	59.9
ndhD_p2	AAATCCAAGAGGTTGCAGCA	CAAATTGCTTCCGCTCCAAG	45.0	50.0	58.0	58.0	1988 bp	0.5	59.9
ndhD_p3	AAATCCAAGAGGTTGCAGCA	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.0	58.0	1943 bp	0.5	59.8
ndhD_p4	AAATCCAAGAGGTTGCAGCA	AAATTGCTTCCGCTCCAAGA	45.0	45.0	58.0	58.1	1987 bp	0.5	59.3
ndhD_p5	AAATCCAAGAGGTTGCAGCA	AATTGCTTCCGCTCCAAGAA	45.0	45.0	58.0	58.1	1986 bp	0.5	59.3