Hotspot

ndhA

Genome · 129308-129860 · 553 bp

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Pi 0.466

MarkerSeek score 73.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 553
variable_sites: 160
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCCAATCAACAACGGGTAA	TACTCCCCACGATCCTGATC	45.0	55.0	58.0	58.0	1492 bp	0.5	59.8
ndhA_p2	TTGCCAATCAACAACGGGTA	TACTCCCCACGATCCTGATC	45.0	55.0	58.0	58.0	1493 bp	0.5	59.8
ndhA_p3	TGTTTGAGGCAAGTCGTCAA	TACTCCCCACGATCCTGATC	45.0	55.0	58.0	58.0	1198 bp	0.5	59.7
ndhA_p4	TTGTTTGAGGCAAGTCGTCA	TACTCCCCACGATCCTGATC	45.0	55.0	58.0	58.0	1199 bp	0.5	59.7
ndhA_p5	TGCCAATCAACAACGGGTAA	AACGAGTGGATGGGTAGGAA	45.0	50.0	58.0	58.1	1627 bp	0.5	59.4