Hotspot

ccsA

Genome · 124012-124953 · 942 bp

Back to Erycibe

Pi 0.462

MarkerSeek score 76.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 942
variable_sites: 295
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTGCAACCTCTTGGATTT	50.0	45.0	57.9	58.0	1170 bp	0.5	59.0
ccsA_p2	TTGGGGGCTAGACTCTTCTC	TGCTGCAACCTCTTGGATTT	55.0	45.0	58.1	58.0	1022 bp	0.5	58.9
ccsA_p3	TAGACACGCTGCTCTTAGGA	GGTATGAGCGGTTTTGTTGC	50.0	50.0	57.9	58.0	1721 bp	0.5	58.8
ccsA_p4	TAGACACGCTGCTCTTAGGA	GTATGAGCGGTTTTGTTGCC	50.0	50.0	57.9	58.0	1720 bp	0.5	58.8
ccsA_p5	TTGGGGGCTAGACTCTTCTC	GTATGAGCGGTTTTGTTGCC	55.0	50.0	58.1	58.0	1572 bp	0.5	58.8