Hotspot

ndhD-psaC

Genome · 126738-126825 · 88 bp

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Pi 0.541

MarkerSeek score 74.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: Genome
length_bp: 88
variable_sites: 40
pi: 0.541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGGCCTACAGAAAGTCCAT	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.0	58.0	971 bp	0.5	59.7
ndhD-psaC_p2	AGGGCCTACAGAAAGTCCAT	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.0	58.0	517 bp	0.5	59.7
ndhD-psaC_p3	GGTCCCGAGAAGCAAATGAT	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.0	58.0	1104 bp	0.5	59.5
ndhD-psaC_p4	AGGGCCTACAGAAAGTCCAT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	472 bp	0.5	59.5
ndhD-psaC_p5	GGTCCCGAGAAGCAAATGAT	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.0	58.0	650 bp	0.5	59.5