Hotspot

rpl32-trnL-UAG

Genome · 122786-123803 · 1018 bp

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Pi 0.426

MarkerSeek score 72.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1018
variable_sites: 228
pi: 0.426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGAGCTTTTGGACAGC	CCCTGGGTAAAAATAGCGCT	50.0	50.0	58.1	57.9	1669 bp	0.5	58.8
rpl32-trnL-UAG_p2	AGGAAAATCCCTTTCGACCG	CCCTGGGTAAAAATAGCGCT	50.0	50.0	57.9	57.9	1631 bp	0.5	58.7
rpl32-trnL-UAG_p3	AGGAAGAGCTTTTGGACAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1168 bp	0.5	58.2
rpl32-trnL-UAG_p4	AGGAAGAGCTTTTGGACAGC	GAGAAGAGTCTAGCCCCCAA	50.0	55.0	58.1	58.1	1316 bp	0.5	58.2
rpl32-trnL-UAG_p5	AGGAAAATCCCTTTCGACCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1130 bp	0.5	58.1