Hotspot

ndhF-rpl32

Genome · 121888-122611 · 724 bp

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Pi 0.425

MarkerSeek score 74.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 724
variable_sites: 190
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCACCGACAAATAATAGCGC	TAAGAGCGAGGCGAGTTCTA	50.0	50.0	58.0	58.0	1720 bp	0.5	59.6
ndhF-rpl32_p2	GCACCGACAAATAATAGCGC	GAAGAGATAAGAGCGAGGCG	50.0	55.0	58.0	58.0	1727 bp	0.5	59.5
ndhF-rpl32_p3	ATCCTAACATGGCAGCACTG	TAAGAGCGAGGCGAGTTCTA	50.0	50.0	58.0	58.0	1422 bp	0.5	59.3
ndhF-rpl32_p4	ATCCTAACATGGCAGCACTG	GAAGAGATAAGAGCGAGGCG	50.0	55.0	58.0	58.0	1429 bp	0.5	59.3
ndhF-rpl32_p5	AGAAGTCAGTGGGTCGATCA	TAAGAGCGAGGCGAGTTCTA	50.0	50.0	58.1	58.0	1292 bp	0.5	59.1