Hotspot

trnQ-UUG

Genome · 5800-5868 · 69 bp

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Pi 0.0551

MarkerSeek score 72.70

Primer available yes

Label trnQ-UUG

Feature Metadata

label_name: trnQ-UUG
feature_type: tRNA
region: Genome
length_bp: 69
variable_sites: 7
pi: 0.0551
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG_p1	CCACCTTTTGAAGAGCCCTT	TGTTGACAAAGACCCATCGG	50.0	50.0	58.0	58.1	1045-1164 bp	1.0	87.5
trnQ-UUG_p2	CCACCTTTTGAAGAGCCCTT	GAATCCTTTCGTCCCAGGTC	50.0	55.0	58.0	58.0	795-827 bp	0.6	65.6
trnQ-UUG_p3	CCACCTTTTGAAGAGCCCTT	CTGTTCTGTATTGGTGCCGA	50.0	50.0	58.0	57.9	736-768 bp	0.6	65.1
trnQ-UUG_p4	CCACCTTTTGAAGAGCCCTT	TGTTCTGTATTGGTGCCGAG	50.0	50.0	58.0	57.9	735-767 bp	0.6	65.1
trnQ-UUG_p5	TGAACAGTTCCTGTGGGTTG	GAATCCTTTCGTCCCAGGTC	50.0	55.0	57.9	58.0	889-921 bp	0.6	65.0