Hotspot

rpl32-rps15

Genome · 105999-107419 · 1421 bp

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Pi 0.0806

MarkerSeek score 76.40

Primer available yes

Label rpl32-rps15

Feature Metadata

label_name: rpl32-rps15
feature_type: igs
region: Genome
length_bp: 1421
variable_sites: 148
pi: 0.0806
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-rps15_p1	GGCGCTCAAAGCTTTTTCAT	CTTCTTGCGAGGTCACTGAA	45.0	50.0	57.9	57.8	364-1558 bp	1.0	90.2
rpl32-rps15_p2	GGCGCTCAAAGCTTTTTCAT	TTCTTCTTGCGAGGTCACTG	45.0	50.0	57.9	57.8	366-1560 bp	1.0	90.2
rpl32-rps15_p3	GGCGCTCAAAGCTTTTTCAT	GTAAGGATGAGCCGGAGAAC	45.0	55.0	57.9	57.8	785-1979 bp	1.0	89.6
rpl32-rps15_p4	GGCGCTCAAAGCTTTTTCAT	AGTAAGGATGAGCCGGAGAA	45.0	50.0	57.9	57.8	1980 bp	0.2	46.2
rpl32-rps15_p5	GGCGCTCAAAGCTTTTTCAT	AGAGTAAGGATGAGCCGGAG	45.0	55.0	57.9	58.3	1982 bp	0.2	45.0