Hotspot

ErcaC_r004-trnR-ACG

Genome · 101766-102005 · 240 bp

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Pi 0.0521
MarkerSeek score 71.70
Primer available yes
Label ErcaC_r004-trnR-ACG

Feature Metadata

label_name
ErcaC_r004-trnR-ACG
feature_type
igs
region
Genome
length_bp
240
variable_sites
19
pi
0.0521
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ErcaC_r004-trnR-ACG_p1 AAAAATAGCTCGACGCCAGG CCACGTGCTCTAATCCTCTG 50.0 55.0 58.6 57.8 283-294 bp 1.0 82.5
ErcaC_r004-trnR-ACG_p2 GGAAAAATAGCTCGACGCCA CCACGTGCTCTAATCCTCTG 50.0 55.0 58.6 57.8 285-296 bp 1.0 82.4
ErcaC_r004-trnR-ACG_p3 GAAAAATAGCTCGACGCCAG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.3 57.8 284-295 bp 1.0 81.7
ErcaC_r004-trnR-ACG_p4 CTGCGGAAAAATAGCTCGAC CCACGTGCTCTAATCCTCTG 50.0 55.0 57.3 57.8 289-300 bp 1.0 81.6
ErcaC_r004-trnR-ACG_p5 TGCGGAAAAATAGCTCGACG CCACGTGCTCTAATCCTCTG 50.0 55.0 59.0 57.8 288-299 bp 1.0 79.4