Hotspot

trnA-UGC-ErcaC_r002

Genome · 97555-98414 · 860 bp

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Pi 0.283

MarkerSeek score 78.20

Primer available yes

Label trnA-UGC-ErcaC_r002

Feature Metadata

label_name: trnA-UGC-ErcaC_r002
feature_type: igs
region: Genome
length_bp: 860
variable_sites: 351
pi: 0.283
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-ErcaC_r002_p1	GGCTGTCAAGAACGTAGAGG	AGTGATGCTTTCGGCTACTG	55.0	50.0	58.0	58.0	1352-1912 bp	0.8	83.9
trnA-UGC-ErcaC_r002_p2	GGGCTGTCAAGAACGTAGAG	AGTGATGCTTTCGGCTACTG	55.0	50.0	58.0	58.0	1353-1913 bp	0.8	83.9
trnA-UGC-ErcaC_r002_p3	GGCTGTCAAGAACGTAGAGG	ATTCGGGAATCTCCGGATCT	55.0	50.0	58.0	58.0	1105-1666 bp	0.8	83.8
trnA-UGC-ErcaC_r002_p4	GGGCTGTCAAGAACGTAGAG	ATTCGGGAATCTCCGGATCT	55.0	50.0	58.0	58.0	1106-1667 bp	0.8	83.8
trnA-UGC-ErcaC_r002_p5	GGCTGTCAAGAACGTAGAGG	ATGTTTCAGTTCGCCAGGTT	55.0	45.0	58.0	58.0	1172-1733 bp	0.8	83.8