Hotspot

trnL-CAA-ndhB

Genome · 87460-88056 · 597 bp

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Pi 0.0382

MarkerSeek score 75.20

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 597
variable_sites: 42
pi: 0.0382
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	ATTCTTGCAATTGCCCAGGA	50.0	45.0	58.0	58.1	661-670 bp	1.0	85.6
trnL-CAA-ndhB_p2	GTCTCGCGTGTCTACCATTT	TTCTTGCAATTGCCCAGGAT	50.0	45.0	58.0	58.1	660-669 bp	1.0	85.6
trnL-CAA-ndhB_p3	CTCGCGTGTCTACCATTTCA	ATTCTTGCAATTGCCCAGGA	50.0	45.0	58.0	58.1	659-668 bp	1.0	85.6
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	TTCTTGCAATTGCCCAGGAT	50.0	45.0	58.0	58.1	658-667 bp	1.0	85.6
trnL-CAA-ndhB_p5	GTCTCGCGTGTCTACCATTT	CGATTCTTGCAATTGCCCAG	50.0	50.0	58.0	58.1	663-672 bp	1.0	85.4