Hotspot

rpl14-rpl16

Genome · 80432-81119 · 688 bp

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Pi 0.146

MarkerSeek score 83.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 688
variable_sites: 171
pi: 0.146
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCCACATTCAAATGGGTCTG	ATGTAGAACCAAAAGAAGATTAG	50.0	30.4	57.3	52.2	723-743 bp	0.6	32.2
rpl14-rpl16_p2	TGTTGTCCGCCACATTCAAA	ATGTAGAACCAAAAGAAGATTAG	45.0	30.4	58.6	52.2	731-751 bp	0.6	32.1
rpl14-rpl16_p3	TTGTCCGCCACATTCAAATG	ATGTAGAACCAAAAGAAGATTAG	45.0	30.4	57.3	52.2	729-749 bp	0.6	32.1
rpl14-rpl16_p4	GTTGTCCGCCACATTCAAAT	ATGTAGAACCAAAAGAAGATTAG	45.0	30.4	57.3	52.2	730-750 bp	0.6	32.1
rpl14-rpl16_p5	CGCCACATTCAAATGGGTCT	ATGTAGAACCAAAAGAAGATTAG	50.0	30.4	58.8	52.2	724-744 bp	0.6	32.1