Hotspot

psbB-psbT

Genome · 73093-73354 · 262 bp

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Pi 0.0487

MarkerSeek score 73.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: Genome
length_bp: 262
variable_sites: 20
pi: 0.0487
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAAAAACTTGGCGATCCA	GGAATGTATAAACCAATGCTTCCA	45.0	37.5	58.0	57.5	287-331 bp	1.0	53.5
psbB-psbT_p2	GGCGATCCAACCACAAAAAG	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.9	57.5	276-320 bp	1.0	53.3
psbB-psbT_p3	ACCACAAAAAGACAGGCAGT	GGAATGTATAAACCAATGCTTCCA	45.0	37.5	57.9	57.5	267-311 bp	1.0	52.9
psbB-psbT_p4	CCAACCACAAAAAGACAGGC	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.8	57.5	270-314 bp	1.0	52.2
psbB-psbT_p5	TCCAAAAACTTGGCGATCCA	AGGAATGTATAAACCAATGCTTCC	45.0	37.5	58.0	57.3	288-332 bp	1.0	51.5