Hotspot

rps12-clpP

Genome · 68288-69623 · 1336 bp

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Pi 0.19

MarkerSeek score 76.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 1336
variable_sites: 323
pi: 0.19
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TCGTAGCATTTCTGACTGGC	CCGCGTATGAAGGACTTTGA	50.0	50.0	58.0	58.0	803-1613 bp	1.0	91.9
rps12-clpP_p2	CCCTCTTAGTCCGTTCTTGC	CCGCGTATGAAGGACTTTGA	55.0	50.0	58.0	58.0	1154-1985 bp	1.0	91.9
rps12-clpP_p3	CGTAGCATTTCTGACTGGCT	CCGCGTATGAAGGACTTTGA	50.0	50.0	58.0	58.0	802-1612 bp	1.0	91.8
rps12-clpP_p4	TCGTAGCATTTCTGACTGGC	GAAAGGGTGGTACGTGGATC	50.0	55.0	58.0	58.0	1034-1842 bp	1.0	91.8
rps12-clpP_p5	CCCTCTTAGTCCGTTCTTGC	GAAAGGGTGGTACGTGGATC	55.0	55.0	58.0	58.0	1385-2214 bp	1.0	91.7