Hotspot

psaI-rpl20

Genome · 65455-67015 · 1561 bp

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Pi 0.0608

MarkerSeek score 79.80

Primer available yes

Label psaI-rpl20

Feature Metadata

label_name: psaI-rpl20
feature_type: igs
region: Genome
length_bp: 1561
variable_sites: 192
pi: 0.0608
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-rpl20_p1	GTTTTTCGAGACCCCGCTAT	TCGGCTCATCGTGATAGAGT	50.0	50.0	58.0	58.0	1372-2256 bp	1.0	91.6
psaI-rpl20_p2	GTTTTTCGAGACCCCGCTAT	TCGTCGTTTGTGGATCACTC	50.0	50.0	58.0	57.9	1334-2218 bp	1.0	91.4
psaI-rpl20_p3	GTTTTTCGAGACCCCGCTAT	TCGCAAAATGCTTGCACAAA	50.0	40.0	58.0	58.1	1226-2110 bp	1.0	91.3
psaI-rpl20_p4	CCAAGATAACTGGAAGCCCC	TCGGCTCATCGTGATAGAGT	55.0	50.0	57.9	58.0	1450-2334 bp	1.0	91.3
psaI-rpl20_p5	TCGAGACCCCGCTATAAGTT	TCGGCTCATCGTGATAGAGT	50.0	50.0	57.9	58.0	1367-2251 bp	1.0	91.2