Hotspot

trnT-UGU-trnL-UAA

Genome · 46686-47673 · 988 bp

Back to Erodium

Pi 0.0272

MarkerSeek score 71.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 988
variable_sites: 48
pi: 0.0272
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GTTGCGTCTACCAATTTCGC	55.0	50.0	57.9	58.1	1012-1121 bp	1.0	85.6
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CGTTGCGTCTACCAATTTCG	55.0	50.0	57.9	57.9	1013-1122 bp	1.0	85.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TCCAATTAAGTCCGTTGCGT	55.0	45.0	57.9	57.8	1025-1134 bp	1.0	85.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TCCGTTGCGTCTACCAATTT	55.0	45.0	57.9	57.8	1015-1124 bp	1.0	85.2
trnT-UGU-trnL-UAA_p5	GACCGTCGCATTACAAATGC	GTTGCGTCTACCAATTTCGC	50.0	50.0	58.2	58.1	1030-1139 bp	1.0	85.1